Protein Expression
Protein expression refers to the way in which proteins are synthesized, modified and regulated in living organisms. In protein research, the term can apply to either the object of study or the laboratory techniques required to manufacture proteins. n practical terms, recombinant protein production depends on using cellular machinery, BOT Bioscience provides four major expression systems-based protein expression techniques.
In prokaryotes, protein expression is termed coupled transcription and translation, because transcription and translation occur simultaneously. The translation of mRNA starts before a mature mRNA transcript is fully synthesized. In eukaryotes, transcription and translation are spatially separated, and occur sequentially with transcription happening in the nucleus and translation, or protein synthesis, occurring in the cytoplasm.
Bioscience protein expression systems include :
E. Coli Protein Expression Service
E. coli is a well-established host that offers short culturing time, easy genetic manipulation and low cost media and expression is fast, with proteins routinely produced in one day. Thus, E. coli has multiple, significant benefits over other expression systems including cost, ease-of-use, and scale. The E. coli protein expression system is suitable for multiple species, acting as a principal tool for recombinant protein expression and biotechnological industrial development, especially for small-protein production. BOT Bioscience extensive expertise in bacterial protein expression enables us to provide you with the best possible support for your research project, whether it is a standard protein or a challenging target.
Yeast Protein Expression Service
Yeast strains are extremely useful for the expression and analysis of recombinant eukaryotic proteins and are ideally suited for large-scale production. Yeast systems also are typically used for structural and functional research such as protein interaction studies. These single-l celled eukaryotic organisms are genetically well characterized and are known to perform many post-translational modifications. They growl quickly in defined medium and are easily adapted to fermentation methods. Yeast systems are also easier and less expensive to work with than insect or mammalian cells. BOT Bioscience extensive expertise in yeast protein expression enables us to provide you with the best possible support for your research project, whether it is a standard protein or a challenging target.
Baculovirus-Insect Protein Expression Service
Insect cells can be used for high level protein expression with modifications similar to mammalian systems. There are several systems that can be used to produce recombinant baculovirus, which can then be utilized to express the protein of interest in insect cells. These systems can be easily scaled up and adapted to high-density suspension culture for large-scale expression of protein that is more functionally similar to native mammalian protein. BOT Bioscience's Baculovirus-Insect protein expression system was developed by our in-house team of scientists to enable virus production and expression of recombinant proteins from baculovirus-infected insect cells. Our high throughput insect expression & purification platform is also available for smallscale production to fit your target screening projects.
Mammalian Protein Expression Service
Mammalian expression systems can be used to produce mammalian proteins that have the most native structure and activity due to itsl physiologically relevant environment. This results in high levels of post-translational processing and functional activity. Mammalian expression systems are the preferred system for the expression of mammalian proteins and can be used for the production of antibodies,l complex proteins and proteins for use in functional cell-based assays. Mammalian expression systems can be used to produce proteins transiently or through stable cell lines, where the expression construct is integrated into the host genome. BOT Bioscience offers recombinant protein expression service in mammalian cells. Our high-level mammalian expression system produces human cell line-derived proteins with full glycosylation, allows you to scale un to 50 liters and request taq remoyal.
Large-Scale Protein Production Service
Large-scale protein production has become a critically important service for applications in the field of proteomics, structural biology and protein therapeutics. Many techniques have been used in the production, including bacterial or eukaryotic cell-based culture cells implementation of fermentation and optimization of pH, O2, temperature and cell density. BOT Bioscience addresses this demand through end-to-end solutions tailored for research and commercial use. Unlock the full potential of your protein research and development with our cutting-edge large-scale protein production services-where high purity meets high volume, and innovation drives success.
Virus-like Particle (VLP) Antigen Production Service
VLP is defined as nanoscale structures made up of assembled viral proteins that lack viral genetic material and non-infectious. So far, the structural proteins from human immunodeficiency virus (HIV), adeno-associated virus, Hepatitis B virus (HBV), Hepatitis C virus (HCV) and bacteriophages are popular used for producing VLPs. BOT Bioscience offers the off shelf VLP productions and services to customize VLP generation and package the VLPs to mature ELISA kit, test standard reagent, superantigens and relevant products. Our experienced scientists and technicians have created a comprehensive platform to generate VLPs supporting both academic and industrial goals and open to discuss the strategy for the cooperation on the cutting edge study.
The introduction of polyethylene glycol (PEG) into biomolecule and biosimilar is well known to enhance the stability and pharmacologicalproperties of proteins, peptides, biosimilar antibody conjugates and small molecule compounds. As a synthetic polymer with high hydrophilicity, PEG is widely used to prolong the circulation time of proteins, increase the aqueous solubility of therapeutic molecules and reduce the immunogenicity of biopharmaceuticals. BOT Bioscience provides generic or site-specific PEGylation ofd proteins, antibodies, aptamers, DNAs, small molecules, dendrimers, nanoparticles, and biodegradable polymers. We also can provide posttranslational polysaccharide modification of biologics, protein lipidation, bioconjugation, and crosslinking chemistry.
Serum-Free Protein Expression Service
Serum-free protein expression refers to the production of recombinant proteins in cell culture systems without the use of animal-derived serum (e.g., fetal bovine serum, FBS). This approach offers significant advantages, including improved reproducibility, reduced risk of contamination by pathogens or immunogenic components, and simplified downstream purification processes. BOT Bioscience provides serum-free expression system services, from the design of serum-free medium to the production of recombinant proteins using serum-free expression systems, comprehensive services can meet the needs of different customers.
SUMO-Tag Protein Production Service
Small- Ubiquitin-like Modifiers, also as known as SUMO proteins, are a proteinfamily composed of various small proteins to modifyfunction of other proteins. SUmocan be covalently attached to other proteins and detached from them in cells,participating invarious cellular processes include signal transduction, nuclear transport, and protein stabilization. BOT Bioscience is rich-experienced in SUMO-tagged protein expression and purification, and we have established a resourceful technical support platform in order to help your custom project.
Endotoxin-Free Protein Production Service
Endotoxins, known as lipoglycans and lipopolysaccharides (LPS), are a part of the outer membrane of the cell wall of Gram-negative bacteria. It is extremely hard to remove them by simple sterilization. Endotoxins can initiate a strong immune response such as endotoxin shock, organ failure, tissue injury, et al. Due to the harmful effects of endotoxin, limiting the protein endotoxin level has become an important direction in the medical field. E. Coli is a preferred system for recombinant protein because it generally provides the most cost effective solution. BOT Bioscience provides cell-based recombinant proteinproduction services that can help you accelerate your discovery in a timely and cost-effective manner at an affordable price.
Stable Cell Line Construction Service
The construction of stable cell line refers to the entry of foreign genes into recipient cells and integration into the chromosomes, allowing the target protein to be stably expressed on the surface of cell membrane. Stable cell line has been used in functional studies of genes, protein production and drug screening. BOT Bioscience has many years of experience in stable cell line construction services. We have established a mature agreement and can provide you with edited stable cell lines according to your requirements.
